spatialepidemiology.net | Bd-Maps

Information


bullet Introduction
bullet Global Mapping
bullet Surveillance
  bullet Global Summary
  bullet Country Summaries
  bullet Species Summaries
  bullet Sequence Type Summaries
bullet Database Interrogation
bullet Private Databases
bullet BLOG
bullet Published articles
bullet Developments in progress
bullet References
bullet Contact
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Introduction

To address the global spread of Bd it is crucial that a dedicated system is available to collect, map and model the prevalence of infection to aid control measures directed against Bd. We have developed this web-based data collection and mapping tool viewable by differing audiences (scientists, policy makers, the public) and aimed at addressing a number of questions (spread, epidemiology, evolution, control).

The overarching aim of Bd-Maps is to provide a truly global, community-focussed resource .All data is presented in a geographical context allowing identification of areas of potential spread and temporal analysis allows previous spread to be modelled and used to identify areas of concern. Multiple private databases are available for users (herein data managers) to enter and analyse their data separately and securely or in conjunction with publicly available data and for such analyses to be directed towards policy makers, resource managers, the general public and the scientific community. All the data on the public site will be downloadable, allowing further analysis and synthesis by interested parties.

Global mapping

A map detailing global incidence of positive Bd collections can be accessed from the frontpage.  Those isolates that have genotypic data are highlighted and views can be toggled.  Clicking on a particular positive site centres and zooms the map and details all data recorded at that location. Listed for each location are the species found (including links to the IUCN Red list1 and the GAA2 to view distribution patterns), method of detection, publication, date of collection and genotype (if known). If genotypic data has been collected, further analysis is provided via phylogenetic trees (based on allelic profiles) and the eBURSTv3 algorithm which groups closely related genotypes together and can be utilised to investigate possible introduction events.
The temporal nature of the data can be analysed using Google Earth, allowing a timeline to be played highlighting where and when positive samples were recorded.

 

Surveillance - see here

Global Summary

Data statistics are available detailing site numbers, countries and species recorded. Countries are ranked based on prevalence and community defined cut-off levels can be set and emails sent to individuals should prevalence/incidence in a particular country exceed the cut-off level.  The same facility is available based on the prevalence of Bd infection is specific species.

More resolved summaries are available as follows: 

1) Country summaries - see here

Any country represented in the database can be viewed through the country summary. Along with a map detailing distribution, summary statistics for sites, samples and species collected, data is returned indicating diagnostic techniques and genotypes if known, which can again be utilised in phylogenetic analysis. Using eBURST, genotypes within the country of choice can be compared to the global population highlighting those genotypes that are found within and outside of the country of choice.  Within country surveillance is undertaken at the species level and country specific-cutoffs can be set by data managers.  Centres of excellence, registers of expertise, testing laboratories and country specific information is also accessible from within these pages and it is envisioned that this will act as a central Bd resource for each country.

2) Species summaries - see here

The global distribution of any species submitted can be viewed. Statistics are viewable indicating number of individuals, number of sites sampled and number of those genotyped. All data for the species is listed below a map of distribution and links to the IUCN and GAA are available on a species basis. Closer linking with the Edge Amphibians website4 is being implemented to provide greater species-specific information, specifically for species that are known to be of priority conservation concern

3) Sequence Type Summaries - see here

Global distribution of an individual genotype can be viewed detailing numbers of genotypes isolates, number of sample sites and closely related genotypes.  As genotyping becomes more widespread this facility will allow the global molecular epidemiology and evolutionary biology of Bd to be investigated and it is envisioned that genotypes will be assigned centrally so that data is comparable between laboratories.  We developed these analytical techniques using the informatic backbone that has proven success in managing the molecular multilocus typing schemes that are in  widespread use in public health investigations of human bacterial and fungal pathogens5

Database interrogation - see here

Standard database interrogation tools are available to return data based on a user entered criteria. For example – all isolates not found in USA which have been confirmed using qPCR.

Private databases

To address data privacy issues and to ensure users have faith in the community nature of the facility, we provide private databases allowing users to enter their own data securely and to have full control over whether this data is kept private or made viewable to the community as a whole. 
Upon registration, a user is provided with a username and password that allows them to login to the curation interface of the website.  Here, an individual (or group) can enter data singly or in bulk.  Single data entry allows single samples or single sites where multiple samples have been collected to be entered.  A user-friendly web form contains data entry fields and where possible we have made dropdown lists available so that data can be entered correctly and easily.  GPS co-ordinates can be entered and facilities for conversion between common formats are available. Should GPS co-ordinates not be available a web-based geocoding facility can be used to lookup location based on addresses or by placing a push-pin on a map – Lat/Lon data is returned for entry into the database.
To address concerns regarding sensitive locational data we have implemented a ‘fuzzy’ location delimiter where a site can be recorded as a particular radius around a point or at any resolution up to country level.  Differential map displays will allow fuzzy data to be aggregated into tessellation maps with different levels of resolution.
A bulk upload facility is available for users who have many data points and do not wish to enter one at a time.  An excel template is available which contains data columns present in the database and suggested values.  This can then be uploaded and viewed online. 

Users can view their data for surveillance purposes with the same summaries as the publicly available data – country summaries, species summaries or genotype summaries.  Furthermore, users can analyse their own data combined with publicly available data.

NB:  All private data is only accessible and full ownership belongs to the user.  The decision to allow data to be viewed publicly is yours.

to request a private database please contact us

BLOG

To disseminate information to the community in a timely fashion the Bd site has a number of associated BLOGS.  The main BLOG can be accessed from the frontpage of the website - http://spatialepidemiology.blogspot.com

Published articles

A list of relevant articles indexed in PubMed is available as an RSS feed from the front page.  We encourage users to let us know of articles not included and / or relevant articles not in published in the scientific literature (Press and media articles, other BLOG posts, newsgroups etc.) Click here

Developments in progress

-The ability to download data and generation of standard reports.
-The inclusion of environmental data and view based maps (eg altitude, average rainfall etc.)
-Real-time modelling to view predictive spread and potential control strategies.
-Comparative genomic analyses are being undertaken to identify the optimum number of polymorphic loci that need to be sequenced to identify the majority of Bd lineages, allowing sensitive and powerful molecular epidemiology and evolutionary studies.
-The inclusion of negative data-points. At the moment, negative data is being collected, but not displayed on the maps. This is because there is not yet a consensus on how many amphibians need to be screened to allow a site to be described as ‘Negative’. The statistical issues of power and sample size, and their graphical representation, are being addressed in a future iteration of the Bd website.

REFERENCES

1)         The World Conservation Unit - http://www.iucn.org/
2)         Global Amphibian Assessment - http://www.globalamphibians.org/
3)         eBURST – http://eburst.mlst.net 
4)         EDGE Amphibians - http://www.edgeofexistence.org/
5)         The multilocus sequence typing network – http://www.mlst.net  

CONTACT

For further details or to register contact - David Aanensen or Matthew Fisher

 

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